Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liu, F.; Yang, H.; Wang, L.; Yu, B.
    Biosynthesis of the high-value plant secondary product benzyl isothiocyanate via functional expression of multiple heterologous enzymes in Escherichia coli (2016), ACS Synth. Biol., 5, 1557-1565 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.8.2.38 synthesis 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae are expressed in Escherichia coli and successfully used for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.24 co-expression of the enzyme with myrosinase from Brevicoryne brassicae in Escherichia coli, expression of codon-optimized sulfotransferase gene SOT16 from Arabidopsis thaliana fails because of the formation of inclusion bodies Arabidopsis thaliana
2.8.2.38 co-expression of the enzyme with myrosinase from Brevicoryne brassicae in Escherichia coli strain BL21(DE3), expression of codon-optimized sulfotransferase gene SOT18 from Arabidopsis thaliana is successful and can substitute for the insoluble SOT16, desulfoglucosinolate:PAPS sulfotransferase Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.24 additional information generation of two chimeric cytochrome P450 enzymes from CYP79A2 and CYP83B1 are constructed and attempted to be functionally expressed in Escherichia coli strain BL21(DE3). The Escherichia coli cystathionine beta-lyase is used to replace the plant-derived CS lyase whose active form cannot be expressed in Escherichia coli. Recomstruction of the BITC biosynthesis pathway from plants in the bacteria, involving CYP79A2 and CYP83B1, and the glutathione S-transferase, gamma-glutamyl peptidase, C-S lyase, glucosyltransferase, desulfoglucosinolate:PAPS sulfotransferase, and myrosinase are encoded by genes GSTF, GGP1, SUR1, UGT74B1, SOT16, and MYR, respectively, overview. Expression of codon-optimized sulfotransferase gene SOT16 from Arabidopsis thaliana fails because of the formation of inclusion bodies, SOT16 is successfully replaced for SOT18 (EC 2.8.2.38) Arabidopsis thaliana
2.8.2.38 additional information generation of two chimeric cytochrome P450 enzymes from CYP79A2 and CYP83B1 are constructed and functionally expressed in Escherichia coli. The Escherichia coli cystathionine beta-lyase is used to replace the plant-derived CS lyase whose active form cannot be expressed in Escherichia coli strain BL21(DE3). Reconstruction of the BITC biosynthesis pathway from plants in the bacteria, involving CYP79A2 and CYP83B1, and the glutathione S-transferase, gamma-glutamyl peptidase, C-S lyase, glucosyltransferase, 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase, and myrosinase are encoded by genes GSTF, GGP1, SUR1, UGT74B1, SOT18, and MYR, respectively, overview Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.24 cytosol
-
 Arabidopsis thaliana 5829
-
2.8.2.38 cytosol
-
 Arabidopsis thaliana 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.24 Arabidopsis thaliana Q9C9D0
-
-
2.8.2.24 Arabidopsis thaliana Col-0 Q9C9D0
-
-
2.8.2.38 Arabidopsis thaliana Q9C9C9
-
-
2.8.2.38 Arabidopsis thaliana Col-0 Q9C9C9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.24 additional information analysis/evaluation of desulfoglucosinolate:PAPS sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro, product identity is confirmed by gas chromatography/mass spectrometry analysis. SOTs from different Arabidopsis ecotypes show different efficiencies Arabidopsis thaliana ?
-
-
2.8.2.24 additional information analysis/evaluation of desulfoglucosinolate:PAPS sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro, product identity is confirmed by gas chromatography/mass spectrometry analysis. SOTs from different Arabidopsis ecotypes show different efficiencies Arabidopsis thaliana Col-0 ?
-
-
2.8.2.38 additional information usage of 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro, product identity is confirmed by gas chromatography/mass spectrometry analysis. SOTs from different Arabidopsis ecotypes show different efficiencies Arabidopsis thaliana ?
-
-
2.8.2.38 additional information usage of 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro, product identity is confirmed by gas chromatography/mass spectrometry analysis. SOTs from different Arabidopsis ecotypes show different efficiencies Arabidopsis thaliana Col-0 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.8.2.24 desulfoglucosinolate:PAPS sulfotransferase
-
 Arabidopsis thaliana
2.8.2.24 SOT16
-
 Arabidopsis thaliana
2.8.2.38 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase UniProt Arabidopsis thaliana
2.8.2.38 cytosolic sulfotransferase 18
-
 Arabidopsis thaliana
2.8.2.38 SOT18
-
 Arabidopsis thaliana